TABLE F.2 Parameters examined in animals of the parental (P) generation. Only statistically significant changes are reported; ‘–’: no statistically significant changes were observed; Dose groups: G1: 0, G2: 16,000, G3: 20,000, G4: 25,000 mg/kg feed. Unless specified differently, the reported values for the respective parameters are within the historical control ranges.

Parameters examined	P generation	P generation
	Males	Females
Mortality	–	–
Clinical signs	–	–
Behavioural examination during lactation	–	–
Body weight during pre‐mating period	Changes in mean body weight and body weight gain, but not dose‐related
Body weight during gestational period	n.a.	Mean gestational body weight on: GD 0, ↓G3 and G4; GD 7 and 14, ↓G2, G3 and G4; GD 20, ↓G2 and G3
Body weight during lactation	n.a.	Mean body weight on: LD 1, ↓G2, G3 and G4; LD 4, ↓G2 and G3; LD 7, 14 and 21, ↓G2, G3 and G4
Feed consumption during pre‐mating period	Mean feed consumption: during week 6, ↑G4	Mean feed consumption: during week 10, ↑G3 and G4
Haematology	Absolute eosinophil count: ↓G3 and G4	–
Clinical biochemistry	–	–
Urinalysis	Mean pH value: ↓G4; Specific gravity: ↑G4	–
Thyroid hormone levels	–	–
Oestrous cycle data	n.a.	Mean oestrus cycle duration: ↑G2 and G4
Sperm parameters	Sperm with abnormalities: ↑G3 and G4	n.a.
Necropsy and gross pathology	–	–
Organ weight	–	Absolute and relative thyroid+parathyroid weights: ↓G3
Histopathology	–	–
Pre‐coital time	–	–, but longer than anticipated for Sprague–Dawley rats
Litter size	n.a.	–, but a dose‐related trend ↓ was observed
Post‐implantation loss	n.a.	–, but a dose‐related trend ↑ was observed

Abbreviations: ‘–’, statistically not significant; ↑, increase; ↓, decrease; G, dose group; GD, gestational period; LD, lactation day; n.a., not applicable.