TABLE 2.

Sequences of primers and characteristics of each nested RT-PCR

Protein gene	Primers	Positions	Sequence (5′→3′)	Cycle profilea	Length of amplified product (bp)
N	N2.1	1–19	ATG GCT CTC AGC AAG GTC A	94°C, 60 s; 58°C, 60 s; 72°C, 90 s	1,034
	N2.2	1034–1013	TCT TGG TTT CTT GGT GTA CCT C
	N2.3	127–146	CAT CTC AAT AAG TTG TGT GG	94°C, 45 s; 49°C, 60 s; 72°C, 60 s	731
	N2.4	857–838	TCT ACA ACC TGT TCC ATT TC
G	G2.5	11–31	AGA CAT TAA AGA GGG CTT GGA	94°C, 60 s; 55°C, 60 s; 72°C, 90 s	1,030
	F2.7	118–99	CTG CAC TGC ATG TTG ATT GA
	VG1	131–155	CCA CCC TAG CAA TGA TAA CCT TGA C	94°C, 45 s; 58°C, 60 s; 72°C, 60 s	541
	VG4	672–648	GCT AGT TCT GTG GTG GAT TGT TGT C
F	F2.1	136–157	AGT GCA TTA AGA ACT GGA TGG T	94°C, 60 s; 58°C, 60 s; 72°C, 90 s	1,335
	F2.2	1470–1450	TGC ATC AAA CTC ATC AGA AGG
	F2.3	433–452	GGA TCT GCT ATT GCA AGT GG	94°C, 45 s; 62°C, 60 s; 72°C, 60 s	833
	F2.4	1265–1244	CAT TTT GTC TTC CCA TAG CAT G

^aThere were 35 cycles. They were preceded by a denaturation step (94°C, 12 min) and followed by an elongation step (72°C, 10 min).