TABLE 2.
Protein gene | Primers | Positions | Sequence (5′→3′) | Cycle profilea | Length of amplified product (bp) |
---|---|---|---|---|---|
N | N2.1 | 1–19 | ATG GCT CTC AGC AAG GTC A | 94°C, 60 s; 58°C, 60 s; 72°C, 90 s | 1,034 |
N2.2 | 1034–1013 | TCT TGG TTT CTT GGT GTA CCT C | |||
N2.3 | 127–146 | CAT CTC AAT AAG TTG TGT GG | 94°C, 45 s; 49°C, 60 s; 72°C, 60 s | 731 | |
N2.4 | 857–838 | TCT ACA ACC TGT TCC ATT TC | |||
G | G2.5 | 11–31 | AGA CAT TAA AGA GGG CTT GGA | 94°C, 60 s; 55°C, 60 s; 72°C, 90 s | 1,030 |
F2.7 | 118–99 | CTG CAC TGC ATG TTG ATT GA | |||
VG1 | 131–155 | CCA CCC TAG CAA TGA TAA CCT TGA C | 94°C, 45 s; 58°C, 60 s; 72°C, 60 s | 541 | |
VG4 | 672–648 | GCT AGT TCT GTG GTG GAT TGT TGT C | |||
F | F2.1 | 136–157 | AGT GCA TTA AGA ACT GGA TGG T | 94°C, 60 s; 58°C, 60 s; 72°C, 90 s | 1,335 |
F2.2 | 1470–1450 | TGC ATC AAA CTC ATC AGA AGG | |||
F2.3 | 433–452 | GGA TCT GCT ATT GCA AGT GG | 94°C, 45 s; 62°C, 60 s; 72°C, 60 s | 833 | |
F2.4 | 1265–1244 | CAT TTT GTC TTC CCA TAG CAT G |
There were 35 cycles. They were preceded by a denaturation step (94°C, 12 min) and followed by an elongation step (72°C, 10 min).