Figure 8.

Detachment of 3-days-old Cutibacterium acnes HL043PA1 biofilms by enzymes and detergents. Biofilms were rinsed with water, treated with the indicated agent for 30 min at 37°C, re-rinsed, and stained with crystal violet. Dispersin B was 50 μg/mL, DNase I was 10 μg/mL, and sodium diodecyl sulfate (SDS) was at 1%. Duplicate tubes for each treatment are shown in the top panel and triplicate tubes for each treatment are shown in the bottom panel. These experiments were performed on three separate occasions with identical results. Representative experiments are shown.