Figure 9.

Dispersin B (DspB) sensitizes Cutibacterium acnes HL043PA1 biofilms to killing by benzoyl peroxide (BP) (A,B) and to growth inhibition by tetracycline (Tet) (C). Panels (A,B) show the effect of a 10-min BP treatment on 72-h-old biofilms cultured in glass tubes. Panel (A) 70 μg/mL BP; panel (B), 140 μg/mL BP. Some biofilms were treated with 20 μg/mL DspB in PBS for 15 min prior to contact with BP as indicated in the legends below. In panel (C), biofilms were cultured for 72-h in 0 or 0.2 μg/mL Tet. Some tubes were supplemented with 100 μg/mL DspB as indicated in the legend below the graph. Each dot represents one individual tube. The experiment in panel (C) was performed on two separate occasions with similarly significant differences between tetracycline alone and tetracycline + dispersin B.