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. 2020 Feb 24;20(2):582–601. doi: 10.5114/aoms.2020.93216

Figure 3.

Figure 3

Figure 3

Influence of TLR4 knock-down on TDSC apoptosis. NC – the TDSCs were treated with normal treatment; Model – the TDSCs were treated with 50 ng/ml TGF-β1; si-NC – the cells were transfected with si-NC (negative control) based on the model; si-TLR4 – the cells were transfected with si-TLR4 (knockdown TLR4) based on the model. A – TLR4 knockdown affect cell apoptosis by flow cytometry. ***P < 0.001, compared with NC group; ##p < 0.01, compared with Model group. B – TLR4 knockdown affect cell apoptosis by TUNEL assay. ***P < 0.001, compared with NC group; ##p < 0.01, compared with Model group