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. 2020 Feb 24;20(2):582–601. doi: 10.5114/aoms.2020.93216

Figure 9.

Figure 9

miRNA-140-5p affects relative protein expression by WB assay. NC – the TDSCs cells were treated with normal treatment; Model – the TDSCs were treated with 50 ng/ml TGF-β1; Vector – the cells were transfected with empty vector based on the model; miRNA-NC – the cells were transfected with miRNA-NC (negative control) based on the model; miRNA – the cells were transfected with miRNA-140-5p based on the model; si-TLR4 – the cells were transfected with si-TLR4 (knockdown TLR4) based on the model; si-TLR4 + miRNA – the cells were transfected with miRNA-140-5p and si-TLR4 (knockdown TLR4) based on the model; miRNA + TLR4 – the cells were transfected with miRNA-140-5p and TLR4 (TLR4 overexpression) based on the model. ***P < 0.001, compared with NC group; ##p < 0.01, compared with miRNA-NC group; $$p < 0.01, compared with miRNA group; ΔΔp < 0.01, compared with si-TLR4 + miRNA group