Fig. 1.

Schematic of the adapted method to isolate proteins from mucin. Adapted method to isolate proteins from mucin (AMIPROM): samples of soft and hard mucin obtained from LG-PMP and HG-PMP are cut into small pieces and homogenised using ultrasound. The homogenate is then centrifuged and the supernatant is collected and filtered. The homogenate is then subjected to liquid chromatography (LC) using two different columns to reduce the most abundant glycoproteins, IgGs, and albumin from the sample. The LC-derived protein extract is subjected to mass spectrometry analysis using an unbiased targeted proteomic approach with SWATH-MS