Fig. 7.

STAT1 facilitates the transcription of HK2. (A) STAT1 was positively correlated with the HK2 expression in LUAD cohort. (B-C) Western blot and PCR results suggested the HK2 expression decreased as STAT1 knockdown. (D) Bioinformation analysis of the promoter binding sites of STAT1. (E) ChIP assay results shown by qPCR indicated the binding of STAT1 on the site2 of HK2 promoter. (F) Agarose gel electrophoresis suggested STAT1 could bind HK2 promoter on site2. (G) Mut and WT sequences of the HK2 promoter site2 were respectively constructed. A luciferase reporter assay was performed to detect the luciferase activity. (H)) Schematic diagram of CCT6A/STAT1/ HK2 axis promoting the glycolysis in LUAD cells. * P < 0.05; ** P < 0.01; *** P < 0.001. Variables are presented as mean ± SD