Figure 2.

Identification of a novel USP7 inhibitor DHPO. A) Overview of in silico screening of small‐molecule inhibitors of USP7. B) Structure of DHPO. C) Molecular docking of DHPO in the allosteric site of human USP7. D) MicroScale Thermophoresis (MST) assay showing binding affinity of DHPO and purified USP7 protein. E) The anti‐proliferative effects of DHPO on MGC803 and MKN1 were assessed using CCK‐8 assay after 72 h of treatment. F) Linear MS spectra of USP7 without or with DHPO incubation show a mass shift of USP7 with the addition of DHPO. G) MS/MS analysis of DHPO‐bound peptide 294–301.