Figure 4.

HSPC bioprinting and differentiation into the 3D silk‐based construct. a), Primary human blood progenitor cells are mixed with the silk bioink and 3D bioprinted into a 3‐layer “flower” construct. The flow cytometry analysis of CD34⁺ cells before 3D bioprinting is shown. Cartoon was made using BioRender.com b), Confocal microscopy analysis of cells after bioprinting (pseudo colors are used to indicate cell distribution; scale bar = 60 µm). c), 3D confocal reconstruction of polyploid megakaryocytes cultured into the silk bioink (green = CD41; blue = nuclei; scale bar = 40 µm, representative of three independent experiments). d), Flow cytometry analysis of the ploidy of megakaryocyte retrieved from the silk bioink using the dissolution buffer (n = 3). e), Percentage of megakaryocytes expressing lineage‐surface markers as assessed by flow cytometry analysis of samples retrieved from the 3D construct (n = 3).