Figure 5.

3D bioprinted silk bioink supports functional platelet production. a), A custom‐made “flower‐holder” was used for performing live‐image analysis (MK = megakaryocyte). b), 3D confocal reconstruction of polyploid megakaryocytes differentiated into the silk bioink (green = CD41; white = nuclei; scale bar = 20 µm) and c), Evaluation of single cell volume and sphericity during differentiation (HSPC = hematopoietic stem and progenitor cell, MK = megakaryocyte; PPF = proplatelet‐forming megakaryocyte, n = 100). d), Spatiotemporal volumetric imaging of samples in the last phase of differentiation shows the process of proplatelet formation. Image segmentation analysis demonstrates that megakaryocytes increase their volume while decreasing sphericity during proplatelet formation. Arrows indicate platelets released from branching filaments (green = CD61; scale bar = 30 µm).