Figure 3.

The HBV and HDV dual channel quantification system can be applied to investigate the interactions between HBV and HDV. (A) HBV DNA detection in the supernatants after HBV single transfection, HBV-HDV co-transfection and HDV-HBV preS1 co-transfection. (B) HDV RNA detection in the supernatants after HBV single transfection, HBV-HDV co-transfection and HDV-HBV preS1 co-transfection. HBsAg (C) and HBeAg (D) detection in the supernatants after HBV single transfection, HBV-HDV co-transfection and HDV-HBV preS1 co-transfection by ELISA. Statistical significance was determined applying an unpaired two-tailed t-test. n = 4. (E) Intracellular HBV core protein and HDV antigen were analysed by western blot after HBV single transfection, HBV-HDV co-transfection and HDV-HBV preS1 co-transfection. Data shown as mean ± SD of 2 independent western blot experiment and the quantification was performed 2 times. Statistical significance was determined applying an unpaired two-tailed t-test.