Figure 3.
IFN-γ levels are positively correlated with increased doses of BCG intradermal challenge. PBMCs were harvested from patients challenged with (A) 2 × 106 (black), (B) 4 × 106 (red), or (C) 8 × 106 (blue) CFUs of BCG, and the level of IFN-γ was quantified via ELISPOT assay following stimulation with Mycobacterium tuberculosis lysate. Open circles represent outliers. Boxes indicate the 25th and 75th percentiles, with the center line indicating the median. Whiskers extend to the 2.5th and 97.5th percentiles. ***P < .001: statistically significant change from baseline (Wilcoxon signed rank test). BCG, Bacillus Calmette-Guérin; CFU, colony-forming unit; IFN-γ, interferon γ; PBMC, peripheral blood mononuclear cell; SFC, spot-forming cell.