Extended Data Fig. 5. Physiological tests of a mock parabiosis procedure to assess the interaction of parabiosis and exercise.

a, Schematic of the experiment to test blood sharing using glucose levels. Glucose is injected into one mouse and subsequently assessed in both mice in mock parabiosis pairs. b, Blood glucose level changes in pairs starting with a young (left) and old (right) glucose bolus. c, Moving distance in cm (left), and velocity (right) in the old isochronic, young isochronic and heterochronic mock parabiosis pairs. Standard error is shown as the error bar. p_distance(Young ISO—Old ISO) = 0.034. d, Movement tracking in the old isochronic, young isochronic and heterochronic mock parabiosis pairs.