Skip to main content
. 2024 May 1;17(4):dmm050471. doi: 10.1242/dmm.050471

Fig. 4.

Fig. 4.

Pink1–Park pathway inhibition attenuated nephrocyte mitochondrial morphological and functional defects. (A–D) Flies used (4-day-old adult females): control (Dot>mito-GFP); park-OE (Dot>mito-GFP,park-OE); park-IR (Dot>mito-GFP,park-RNAi); Pink1-OE (Dot>mito-GFP,Pink1-OE); Pink1-IR (Dot>mito-GFP,Pink1-RNAi). (A) Mitochondrial morphology visualized by UAS-mito-GFP induced by nephrocyte-specific driver Dot-Gal4. Mitochondrial membrane potential detected through TMRM fluorescence. Scale bar: 1 µm. (B) Quantification of mitochondrial size in nephrocytes from flies on a high-sucrose diet and a normal sucrose control. n=size 30 mitochondria per nephrocyte averaged, for 30 nephrocytes from six flies per group. Results show mean±s.d. normalized to the control group. *P<0.05 [Shapiro–Wilk test (α=0.05) indicated high-sucrose data not normally distributed; Kruskal–Wallis H-test followed by Dunn's test; normal versus high, P=3.012×10−11; high versus high+park-IR, P=7.364×10−10; high versus high+Pink1-IR, P=3.681×10−11]. (C) Quantification of mitochondrial TMRM intensity in nephrocytes that overexpressed or inhibited park or Pink1, from flies on a high-sucrose diet relative to nephrocytes from control flies (Dot>mito-GFP) on a high-sucrose or normal sucrose diet. n=mitochondria in 30 nephrocytes from six flies per group. Results show mean±s.d. normalized to the control group. *P<0.05 [Shapiro–Wilk test (α=0.05) indicated normal sucrose and high-sucrose plus Pink1-OE data were not normally distributed; Kruskal–Wallis H-test followed by Dunn's test; normal versus high, P=3.02×10−11; high versus high+park-IR, P=5.5×10−10; high versus high+Pink1-IR, P=3.02×10−11]. (D) Quantification of ATP level in flies on a high-sucrose diet and a normal sucrose control. n=6 flies per group. Results show mean±s.d. normalized to the control group. *P<0.05 [Shapiro–Wilk test (α=0.05) indicated normal distribution; one-way ANOVA followed by Tukey–Kramer post-test; normal versus high, P=0.0001341; high versus high+park-IR, P=0.0003784; high versus high+Pink1-IR, P=0.0001346].