FIG 4.

Paired box plots showing changes in (A) CD3e⁺, (B) CD4⁺, (C) CD8a⁺, and (D) PD-1⁺ T cells in patients with pre- and post-ICI tumor samples that underwent multiplexed immunofluorescence. (E) Paired box plot showing a significant decrease in PD-1–PD-L1 engagement at the time of AR to ICI. Representative case of a patient with (F) pre- and (G) post-immunotherapy multiplexed immunofluorescence showing significant decrease in tumor-infiltrating CD4 and CD8a T cells. (H) Paired box plots showing significant increase in the neighbor distance between tumor cells (cytokeratin+) and CD8⁺PD-1⁺ T cells at the time of AR to immunotherapy. Representative ray plots showing increase in the neighbor distance between tumor cells (cytokeratin+) and CD8⁺PD-1⁺ T cells between (I) pre- and (J) post-ICI tumor samples. AR, acquired resistance; ICI, immune checkpoint inhibitor.