FIG. 1.

Telomerase activity in stable HFF clones. Stable HFF clones obtained by transfection with pGRN145 expressing the catalytic subunit of telomerase (Tel) were analyzed for telomerase activity by the TRAP assay using a TRAPEZE kit (Intergen) according to the manufacturer's instructions. Two thousand cells were analyzed in all samples. TRAP products were separated by gel electrophoresis (11.25% polyacrylamide) and detected by autoradiography. As a negative control, cell lysates were incubated at 85°C for 10 min (ΔH) to inactivate telomerase. TSR8, positive control for the PCR; IC, internal control for all reactions.