Fig. 3. Inter-subunit coordination of tRNA binding.
a Close-up views of tRNA recognition by ELP1 and ELP3. The density of the full tRNA is shown while the three major contacts are displayed. The close-up views of contacting residues with tRNA interaction are shown on the right. Sharpened map of ELP3 in blue and unfiltered map of ELP1 in gray. b Left: MST measurements with calculated Kd values for ELP123 and ELP3 mutants bound to tRNAGlnUUG. n = 3 (independent experiments). Right: Acetyl-CoA hydrolysis rates of ELP123 and ELP1 and ELP3 mutants in the presence of tRNAGlnUUG, n = 3 (independent experiments). Statistical analysis: one-way ANOVA with Dunnett’s multiple comparisons test. Statistically significant differences are indicated. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.