Table 1.
Detection and diagnostic methods for FCV infection.
| Detection target | Assay type | References |
|---|---|---|
| Nucleic acids | (Triplex) TaqMan real-time reverse transcription-PCR | Abd-Eldaim et al. (2009), Cao et al. (2021) |
| (Multiplex) RT-PCR | Scansen et al. (2005), Kim et al. (2020) | |
| RT-qPCR (or with fluorescence resonance energy transfer probe) | Helps and Harbour (2003), Wilhelm and Truyen (2006), Meli et al. (2018), Phongroop et al. (2023) | |
| CRISPR-Cas13a based visual detection | Huang et al. (2022) | |
| Enzymatic recombinase amplification combined with a lateral flow dipstick | Liu et al. (2022) | |
| Multiplex RT-PCR/PCR | Sykes et al. (2001) | |
| Dual/triple Nano-PCR | Ye et al. (2022), Yan et al. (2023) | |
| Virus particles | CrFK cell-based plaque assay | Bidawid et al. (2003) |
| Carboxymethyl-cellulose plaque assay | Escobar-Herrera et al. (2007) | |
| Antigens or antibodies | Immunohistochemistry | Pesavento et al. (2004), Balboni et al. (2022), Fontes et al. (2023) |
| Enzyme-linked immunosorbent assay (ELISA) for antigens | Tajima et al. (1998) | |
| Point-of-care ELISA for protective antibodies | Digangi et al. (2011) | |
| Double-antibody sandwich ELISA for antigens | Yuan et al. (2014) | |
| Surface plasmon resonance biosensor for antigens | Yakes et al. (2013) |