Figure 4.

Downregulation of SALL3 drives the impaired neuroectoderm differentiation of hESCs with 18q loss

(A) Relative mRNA expression for PAX6. Data are shown as means ± SEMs. Each data point refers to an independent differentiation experiment, and ^∗, ^∗∗, ^∗∗∗, and ^∗∗∗∗ represent statistical significance between samples at 5%, 1%, 0.1%, and 0.01%, respectively (unpaired t test).

(B) Immunostaining for PAX6 and POU5F1 in mutant and control lines. Scale bars (KD groups), 50 μm, and magnification 20 μm; scale bars (OE groups), 100 μm, and magnification, 50 μm.

(C) Percentage of PAX6⁺ cells in the immunostainings shown in (B).