Figure 5.

Changes in SALL3 expression do not regulate cardiac progenitor differentiation

(A) Relative mRNA expression for GATA4. Data are shown as means ± SEMs. Each data point refers to an independent differentiation experiment, and ^∗, ^∗∗, ^∗∗∗, and ^∗∗∗∗ represent statistical significance between samples at 5%, 1%, 0.1%, and 0.01%, respectively (unpaired t test).

(B) Immunostaining for GATA4 and POU5F1 in mutant and control lines. Scale bars (original images), 100 μm, and scale bars (magnification images), 20 μm. (C) Percentage of GATA4⁺ cells in the immunostainings shown in (B).