Fig. 4.

ACSL4 overexpression favors healthier mitochondria with active respiration in MCF-7 breast cancer cells. Mitochondrial respiratory activity and health evaluated in MCF-7 tet-off/ACSL4 and MCF-7 tet-off control cells. (A) Fluorescence microscopy images of mitochondria stained with MTR (red) and nuclei stained with DAPI (blue). Images were obtained at equal exposure time and intensity. (B) Quantification of integrated MTR signal as reference of mitochondrial activity. Results are expressed as the mean integrated signal relative to number of cells ± SEM of at least three independent experiments (***p < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)