Fig. 8.

ACSL4 overexpression reduces mitochondrial mass in MCF-7 breast cancer cells. Mitochondrial mass analysis in MCF-7 tet-off/ACSL4 and MCF-7 tet-off control cells. (A) Fluorescence microscopy images of cells stained with anti-TOM20 (green), and nuclei stained with DAPI (blue). Images were obtained at equal exposure time and intensity. (B) Quantification of TOM20 immunofluorescence. (C) Representative histogram of MTG staining as a measure of mitochondrial mass. Fluorescence intensity was measured at 516 nm emission (FL-1). (D) Quantification of MTG signal is expressed as mean fold change of integrated signal density per number of cells ± SEM of at least three independent experiments (*p < 0.05). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)