Fig. 8.

RNA sequencing of the osteochondral regeneration samples at week 12. A) Principal component analysis (PCA) of the osteochondral tissue transcriptome from the Normal, G-dNC/nHA, Control groups. B) heatmap of the differentially expressed genes (DEGs) in the G-dNC/nHA group and the Control group. C, D) Volcano diagram of G-dNC/nHA vs. control (negative control) groups and G-dNC/nHA vs. normal (positive control) groups. E, F) Relative expression levels of chondrogenesis and osteogenesis of G-dNC/nHA and control groups. G) Gene ontology enrichment and H) Kyoto Encyclopedia of Genes and Genomes enrichment analysis of up-regulated genes of the G-dNC/nHA group relative to the control group. All data were analyzed by a t-test analysis and error bars indicate Mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.“ns” represents no significance.