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. 2024 May 3;8:82–89. doi: 10.5414/ALX2483E

Table 2. Recommendations for the preparation of common occupational materials for the patch test.

Substance Concentration Vehicle pH* Remark
Hand and skin disinfectant Pure
(50%)
Water
Surface disinfectant
(use concentration)
0.1%
0.01%
Water
Instrument disinfectant
(use concentration)
0.01%
(0.001%)
Water
Metalworking fluid, water-soluble, used or unused concentration ≤ 8% Pure
(50%)
Water X 24-hour occlusion
Store sample in a cool place
pH not > 9.5
Metalworking fluid, water-soluble, used or unused concentration > 8% 50%
(10%)
Water X 24-hour occlusion
Store sample in a cool place
pH not > 9.5
Metalworking fluid, water-soluble, concentrate 5% Water X 24-hour occlusion
pH not > 9.5
Metalworking fluid, oily 50%
(10%)
Olive oil
Grease Pure
(20%)
Petrolatum
Lubricating oil 50%
10%
Olive oil
Gear oil 10% Olive oil
Hydraulic oil (50%)
10%
Olive oil
Silicone spray 10% Petrolatum
Wood dust Pure moistened Exotic woods 1 : 10 petrolatum

*pH value must be checked before testing (pH value 5 – 8 is unproblematic).