Figure 10.

Effects of drug treatments on ambulation and water intake in mice with conditional deletion of CB₁Rs in the intestinal epithelium fed Western diet (WD). A, B, Total distance traveled and, C, D, cumulative water intake was measured by automated feeding chambers for a 24 h period starting at the onset of the dark cycle (1,800 h) following a single IP injection of AM6545 (10 mg/kg) or ATR (2 mg/kg) in intCB₁+/+ and intCB₁−/− fed WD. A, A single dose of AM6545 in IntCB₁+/+ controls affected distance traveled across the entire 24 h testing period. ATR also reduced distance traveled in the same mice at the 1 and 24 h timepoints. B, There was a significant effect of drug and drug × time interaction in IntCB₁−/− mice on distance traveled, but the Holm–Sidak’s multiple-comparisons post hoc analysis did not reveal any significant differences at individual time points. C, Water intake of intCB₁+/+ mice was not significantly affected by either drug treatment for the 24 h test. D, There was a significant effect of drug, as well as a drug × time interaction on water intake inintCB₁−/− animals. Specifically, AM6545 treatment significantly reduced cumulative water intake at the 1, 6, and 24 h timepoints. two-way ANOVA followed by Holm–Sidak's multiple-comparisons test when appropriate, see Table 2 for detailed statistics. All data are presented as mean ± SEM, n = 11 or 16 (intCB₁+/+ and intCB₁−/−, respectively); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.