Figure 2.
Mice fed with Western diet (WD) become obese and hyperphagic. A, Body weight was recorded bi-weekly between 0900 and 1,000 h (time × diet interaction, F(16,480) = 121.8; p < 0.0001; diet main effect F(1,30) = 79.56; p < 0.0001; two-way ANOVA followed by Holm–Sidak's multiple-comparisons test). B, Change in body mass (time × diet interaction, F(16,480) = 121.8; p < 0.0001; diet main effect F(1,30) = 195.4; p < 0.0001; two-way ANOVA followed by Holm–Sidak's multiple-comparisons test). C, Total caloric intake during a 24 h test period (t(30) = 3.666; p = 0.0009; unpaired Student's t test). D, At the end of the 60 d diet exposure period to Western diet (WD), epididymal fat pads were weighed (t(30) = 9.686; p > 0.0001; unpaired Student's t test). All data are presented as mean ± SEM, n = 16 per diet; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.