Figure 6.

Anticholinergics do not affect 2-AG metabolic enzyme activity ex vivo. Activity of DGL in the upper SI epithelium from mice fed with Western diet (WD) was assayed in the presence increasing concentrations of a DGL-specific inhibitor and various mAChR antagonists. A, DGL activity was inhibited in a concentration-dependent manner when incubated with THL at concentrations ranging from 3–1,000 nM (IC₅₀ = 58.52 nM, R² = 0.9499). B, DGL activity was not directly inhibited by ATR at concentrations ranging from 10–10,000 nM (R² = −0.0212). C, DGL activity was not directly inhibited by DAU at concentrations ranging from 10–10,000 nM (R² = −0.0286). D, DGL activity was not directly inhibited by PIR at concentrations ranging from 10–10,000 nM (R² = −0.0113). All data are presented as mean ± SEM, n = 3 animals per drug. All graphs are least squares fit of log[inhibitor] versus normalized response.