Figure 9.

Inhibiting peripheral CB₁Rs or mAChRs failed to affect food intake in mice conditionally lacking CB₁Rs in the intestinal epithelium. A, AM6545 (10 mg/kg) or ATR (2 mg/kg) reduced caloric intake for up to 24 h in control intCB₁+/+ mice (time × drug interaction, F_(6,79)= 5.099; p = 0.0002; drug main effect F_(2,30)= 6.024; p = 0.0063; 12 h vehicle vs 12 h AM6545 p = 0.0498, 24 h vehicle vs 24 h AM6545 p = 0.0012, 24 h vehicle vs 24 h ATR p = 0.0043, two-way ANOVA followed by Holm–Sidak's multiple-comparisons test). B, AM6545 or ATR did not affect caloric intake in intCB₁−/− mice (time × drug interaction, F_(6,135)= 0.7700; p = 0.5948; drug main effect F_(2,45)= 0.9273; p = 0.4030; two-way ANOVA). C, Body weights were similar between intCB₁−/− when compared to intCB₁+/+ mice control mice fed with Western diet (WD; time × genotype interaction, F_(9,225)= 5.327; p < 0.0001; genotype main effect F_(1,25) = 0.01602; p = 0.9003; two-way ANOVA followed by Holm–Sidak's multiple-comparisons test). D, Change in body weight was lower in intCB₁−/− when compared to intCB₁+/+ control mice (time × genotype interaction, F_(9,225)= 5.327; p < 0.0001; genotype main effect F_(1,25) = 5.077; p = 0.0333; two-way ANOVA followed by Holm–Sidak's multiple-comparisons test). All data are presented as mean ± SEM, n = 16, 11 (intCB₁−/−, intCB₁+/+ respectively), p < 0.05, **p < 0.01.