Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 16;22:277. doi: 10.1186/s12964-024-01647-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Modulation of in vitro cell migration by YAP silencing in Bcl-2 overexpressing melanoma clones. A Representative images and B relative quantification of in vitro cell migration of control (Control) and Bcl-2 overexpressing A375 (Bcl-2/1) clones and Bcl-2/1 clone transiently transfected with si-YAP (Bcl-2/1 si-YAP) or with control siRNA (Bcl-2/1 si-Ctrl). The values are reported as ratio (mean ± standard deviation) of number of migrated cells/field versus control. The quantification was performed by counting the number of migrated cells in at least 5 fields for each condition. C qRT-PCR analysis to evaluate efficiency of YAP silencing in A375 Bcl-2/1 cells transiently transfected with siRNA SMARTpools targeting YAP (si-YAP) or with control siRNA (si-Ctrl). D Representative images and E relative quantification of in vitro ability of A375 control and A375 Bcl-2/1 clones and A375 Bcl-2/1 clone transiently transfected with si-YAP or with control si-Ctrl cells to migrate in wound healing assay. The values are reported as ratio (mean ± standard deviation) respect to control. A-E Experiments have been conducted in biological triplicate, except for B (biological duplicate is reported). Statistical analysis was performed applying unpaired two-tailed student’s t test, *p < 0.05, **p < 0.01, ***p < 0.01, #p = 0.05, ns = not significant