Fig. 7.

Regulation of melanoma cell viability by Bcl-2 in different stiffness conditions. A Representative images and B relative quantification of viability of A375 cells plated on different stiff conditions, 1 kPa and 50 kPa, and of A375 cells plated on 50 kPa and transiently transfected with siRNA SMARTpools targeting Bcl-2 (si-Bcl-2) or with control siRNA (si-Ctrl). The values are reported as number (mean ± standard deviation) of viable cells. C Representative images and D relative quantification of viability of A375 Control and Bcl-2 overexpressing (A375 Bcl-2/1) clones seeded on 1 kPa and 50 kPa stiffness. The values are reported as ratio (mean ± standard deviation) of viable A375 Bcl-2/1 cells over control. E Representative images and F relative quantification of viability of A375 Bcl-2/1 cells seeded on 50 kPa stiffness and transiently transfected with siRNA SMARTpools targeting YAP (si-YAP) or with control siRNA (si-Ctrl). The values are reported as ratio (mean ± standard deviation) of viable cells over control. B, D, F Cell viability was assessed by Trypan blue staining and using an automatic cell counter. A-F Experiments have been performed in three biological replicates. Statistical analysis was performed applying unpaired two-tailed student’s t test, *p < 0.05; ns = not significant