Fig. 8.

Fibroblast activation by Cultured Medium (CM) from melanoma cells in a Bcl-2-dependent manner. A Representative images and B quantification of human foreskin fibroblasts (HFF) migration in response to CM from control (CM M14 Control) or Bcl-2 overexpressing (CM M14 Bcl-2/6) M14 clones and control (CM A375 Control) or Bcl-2 overexpressing (CM A375 Bcl-2/1) A375 clones. The values are reported as ratio (mean ± standard deviation) of migrated cells/field respect to control. The quantification was performed by counting the number of migrated cells in at least 3 fields for each condition. C Western blot and relative densitometric analysis of α-SMA, pERK1/2 and ERK1/2 proteins in HFF stimulated for 24 h with CM from Control or Bcl-2/6 M14 clones. One representative western blot analysis out of three with similar results is reported. HSP72/73 is shown as loading and transferring control. D Representative images and E quantification of HFF stably expressing GFP protein in co-culture with M14 Control and M14 Bcl-2/6 cells. The values are reported as ratio (mean ± standard deviation) of GFP positive cells in HFF/M14 Bcl-2/6 cells co-culture respect to HFF/M14 control cells co-culture. A-E Experiments have been conducted in triplicates. Statistical analysis was performed applying unpaired two-tailed student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001