Fig. 9.

YAP inhibition by Verteporfin reverts fibroblast activation. A Representative images and B quantification of human foreskin fibroblasts (HFF) migration in response to Conditioned Medium (CM) from Bcl-2 overexpressing M14 (Bcl-2/6 M14) cells treated with Verteporfin (VP, 1µM, 24 h) or vehicle. The values are reported as ratio (mean ± standard deviation) of migrated cells/field respect to control. The quantification was performed by counting the number of migrated cells in at least 3 fields for each condition. C Western blot and densitometric analyses of α-SMA, pERK1/2 and total ERK1/2 proteins in HFF stimulated for 24 h with CM from Bcl-2/6 M14 cells in presence or absence of VP. One representative western blot analysis out of two with similar results is reported. HSP72/73 is shown as loading and transferring control. A-C Experiments have been conducted in triplicates. Statistical analysis was performed applying unpaired two-tailed student’s t test, *p < 0.05