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. 2024 May 16;22:255. doi: 10.1186/s12951-024-02509-1

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© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Characterization of extracellular vesicles isolated from human ASCs. (A) Size distribution of EVs isolated from healthy ASCs (H) or ETO-induced senescent ASCs (S) by Nano Tracking Analysis. The median and modal sizes of EVs are shown in the middle and right panels, respectively (n = 3). (B) EV numbers produced by 1 million ASCs per day (left panel) (n = 3) or EV equivalent in µg of proteins produced by 1 million ASCs (right panel) (n = 20–23). (C) Quantity of total proteins (left panel) and total RNA (right panel) produced per particle (n = 3). (D) Expression profile of membrane markers on the surface of H-EVs (black line), S-EVs (Blue line) or isotypic control (grey histogram) by FACS. (E) Representative pictures of H-EVs and S-EVs by cryo-transmission electron microscopy (scale bar: 100 nm). Data are shown as mean ± SEM. Statistical analysis used the Mann-Whitney test. *p < 0.05, ****p < 0.0001