TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and phenotype | Source or reference(s) |
|---|---|---|
| E. coli | ||
| SM10λpir | thi recA thr leu tonA lacY supE | Laboratory strain |
| RP4-2-Tc::Mu λ::pir | ||
| DH5αλpir | F− Δ(lacZYA-argF)U169 recA1 | 11, 18 |
| endA1 hsdR17 supE44 | ||
| thi-1 gyrA96 relA1 λ::pir | ||
| V. cholerae | ||
| C6709-1 | El Tor biotype, Smr | 33 |
| AC-V66 | C6709-1 lacZ::res1-tet-res1 Tcr LacZ− | 5 |
| AC-V168 | C6709-1 lacZ::pGP704, LacZ− | 5 |
| DSM-V376 | AC-V66 cadA::pGP704 | 25 |
| DSM-V387 | C6709-1 cadB::pGP704 | This work |
| DSM-V591 | C6709-1 cadC::pGP704 | This work |
| DSM-V783 | C6709-1 ΔcadBA | This work |
| Plasmids | ||
| pAC212 | pGP704::SfiI-neo-SfiI | 25 |
| pDSM-373 | pGP704::cadB′ | This work |
| pDSM582 | pCR-script Amp SK(+)::′cadC′ | This work |
| pDSM588 | pGP704::′cadC′ | This work |
| pDSM667 | pGemT::cadBA | This work |
| pDSM673 | pGemT::cadC′ | This work |