Table 1.
Differentially abundant proteins identified in RICTOR-deficient M14 cells by proteomic analysis. Column 1 reports the spot numbers corresponding to those indicated in the representative image of Figure S6. Protein names of the identified spots by MALDI-ToF MS, corresponding UniProt gene names, fold change expressed as the ratio between M14 shR1 and shC (means of the Volumes of single spots/Volume of total spots quantified by densitometric analysis), and the p-value determined by one-way ANOVA Test (n = 5) are indicated
| Spot n° | Protein name | Gene name | Fold change (shR/shC) | p-value |
|---|---|---|---|---|
| 1 | Syntenin-1 | SDCBP | 1.64 | 0.0010 |
| 2 | 3-hydroxyacyl-CoA dehydrogenase type-2 | HSD17B10 | 1.97 | 0.0011 |
| 3 | Nicotinamide phosphoribosyltransferase | NAMPT | 1.54 | 0.0014 |
| 4 | Peroxiredoxin-5, mitochondrial | PRDX5 | 1.59 | 0.0025 |
| 5 | Nicotinamide phosphoribosyltransferase | NAMPT | 1.87 | 0.0027 |
| 6 | Glutamine amidotransferase-like class 1 domain-containing protein 3, mitochondrial | GATD3 | 1.55 | 0.0028 |
| 7 | Histone H2A type 1-H | H2AC12 | 1.67 | 0.0031 |
| 8 | Triosephosphate isomerase | TPI1 | 1.80 | 0.0040 |
| 9 | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial | PDHA1 | 2.58 | 0.0044 |
| 10 | UBX domain-containing protein 1 C-term fragment | UBXN6 | 2.69 | 0.0049 |
| 11 | Transgelin-2 | TAGLN2 | 1.83 | 0.0052 |
| 12 | High mobility group protein B1 | HMGB1 | 1.52 | 0.0070 |
| 13 | Poly(rC)-binding protein 1 | PCBP1 | 2.66 | 0.0072 |
| 14 | Pirin | PIR | 1.50 | 0.0097 |
| 15 | Small ribosomal subunit protein eS12 | RPS12 | 1.59 | 0.0104 |
| 16 | Polyubiquitin-B [free Ubiquitin] | UBB | 1.67 | 0.0104 |
| 17 | Phosphoserine phosphatase | PSPH | 2.77 | 0.0110 |
| 18 | Triosephosphate isomerase | TPI1 | 1.77 | 0.0124 |
| 19 | Superoxide dismutase [Mn], mitochondrial | SOD2 | 1.73 | 0.0124 |
| 20 | Nicotinate phosphoribosyltransferase | NAPRT | 1.56 | 0.0125 |
| 21 | Prelamin-A/C | LMNA | -1.73 | 0.0132 |
| 22 | F-box only protein 22 | FBXO22 | 2.90 | 0.0158 |
| 23 | Cofilin-1 | CFL1 | 1.51 | 0.0173 |
| 24 | Cold shock domain-containing protein E1 | CSDE1 | -1.56 | 0.0174 |
| 25 | Aldo-keto reductase family 1 member A1 | AKR1A1 | 1.53 | 0.0182 |
| 26 | Annexin A11 | ANXA11 | 2.48 | 0.0188 |
| 27 | Sialic acid synthase | NANS | 1.70 | 0.0204 |
| 28 | Far upstream element-binding protein 1 | FUBP1 | -2.81 | 0.0216 |
| 29 | Translation initiation factor eIF-2B subunit alpha | EIF2B1 | 1.54 | 0.0243 |
| 30 | Proteasome subunit alpha type-2 | PSMA2 | 1.58 | 0.0250 |
| 31 | Endoplasmic reticulum resident protein 44 | ERP44 | 1.60 | 0.0258 |
| 32 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | NDUFS1 | 4.17 | 0.0260 |
| 33 | Tubulin alpha-1C chain | TUBA1C | 1.51 | 0.0272 |
| Peptidyl-prolyl cis-trans isomerase FKBP4 | FKBP4 | |||
| 34 | CCHC-type zinc finger nucleic acid binding protein | CNBP | 2.09 | 0.0314 |
| 35 | Scinderin | SCIN | 1.54 | 0.0344 |
| 36 | Protein S100-A4 | S100A4 | -3.33 | 0.0366 |
| 37 | Alpha-centractin | ACTR1A | 1.62 | 0.0391 |
| Elongation factor Tu, mitochondrial | TUFM | |||
| 38 | Inosine-5'-monophosphate dehydrogenase 2 | IMPDH2 | 1.56 | 0.0393 |
| Adenylyl cyclase-associated protein 1 | CAP1 | |||
| 39 | Galectin-3 | LGALS3 | 1.60 | 0.0451 |
| 40 | MYG1 exonuclease | MYG1 | 2.31 | 0.0494 |