Table 2.
Differentially abundant proteins identified in RICTOR-deficient A375 cells by proteomic analysis. Protein names of the identified spots by MALDI-ToF MS, corresponding UniProt gene names, fold change expressed as the ratio between A375 shR1 and shC (means of the Volumes of single spots/Volume of total spots quantified by densitometric analysis), and the p-value determined by one-way ANOVA Test (n = 5) are indicated
| Spot n° | Protein name | Gene Name | Fold change (shR/shC) | p-value |
|---|---|---|---|---|
| 1 | Peptidyl-prolyl cis-trans isomerase A | PPIA | -2.29 | 0.0101 |
| 2 | Histone H2B type 1-B | H2BC3 | -2.25 | 0.0253 |
| 3 | Hippocalcin-like protein 1 | HPCAL1 | -3.97 | 0.0342 |
| 4 | GTP-binding nuclear protein Ran | RAN | 2.69 | 0.0032 |
| 5 | Eukaryotic translation initiation factor 6 | EIF6 | -2.84 | 0.0138 |
| 6 | Proteasome subunit alpha type-3 | PSMA3 | -3.35 | 0.0034 |
| 7 | 6-phosphogluconolactonase | PGLS | -2.42 | 0.0062 |
| 8 | Proteasome activator complex subunit 3 | PSME3 | -2.24 | 0.0217 |
| 9 | Serine/arginine-rich splicing factor 1 | SRSF1 | -2.67 | 0.0120 |
| 10 | Tubulin beta chain | TUBB | 3.66 | 0.0055 |
| 11 | Cathepsin Z | CTSZ | -3.50 | 0.0001 |
| 12 | Serine-threonine kinase receptor-associated protein | STRAP | 12.03 | 0.0215 |
| 13 | Twinfilin-2 | TWF2 | -2.89 | 0.0029 |
| 14 | Actin, cytoplasmic 1 | ACTB | -2.63 | 0.0223 |
| 15 | Ubiquilin-1 | UBQLN1 | -2.34 | 0.0016 |
| 16 | Glycine–tRNA ligase | GARS1 | -2.14 | 0.0370 |
| 17 | Eukaryotic translation initiation factor 4B | EIF4B | -2.61 | 0.0062 |
| 18 | Eukaryotic translation initiation factor 4B | EIF4B | -2.43 | 0.0008 |
| 19 | Far upstream element-binding protein 2 | KHSRP | 3.81 | 0.0021 |
| 20 | Elongation factor 2 | EEF2 | 2.61 | 0.0222 |
| 21 | Glycogen phosphorylase, brain form | PYGB | 2.09 | 0.0336 |
| Elongation factor 2 | EEF2 | |||
| 22 | Vinculin | VCL | 2.14 | 0.0061 |
| 23 | Vinculin | VCL | 2.55 | 0.0013 |
| 24 | Protein S100-A10 | S100A10 | -1.66 | 0.0106 |
| 25 | Peptidyl-prolyl cis-trans isomerase A | PPIA | 1.55 | 0.0281 |
| 26 | Eukaryotic translation initiation factor 5A-1 | EIF5A | -1.61 | 0.0022 |
| 27 | Chromobox protein homolog 3 | CBX3 | -1.58 | 0.0222 |
| 28 | Prohibitin 1 | PHB1 | -1.58 | 0.0094 |
| 29 | Calpain small subunit 1 | CAPNS1 | -1.61 | 0.0368 |
| 30 | Isopentenyl-diphosphate Delta-isomerase 1 | IDI1 | 1.63 | 0.0053 |
| 31 | 26S proteasome non-ATPase regulatory subunit 14 | PSMD14 | 1.46 | 0.0236 |
| 32 | Large ribosomal subunit protein uL10 | RPLP0 | 1.47 | 0.0107 |
| 33 | Crk-like protein | CRKL | -1.64 | 0.0381 |
| 34 | Elongation factor Tu, mitochondrial | TUFM | 1.53 | 0.0332 |
| 35 | Anamorsin | CIAPIN1 | -1.56 | 0.0375 |
| 36 | 26S proteasome non-ATPase regulatory subunit 13 | PSMD13 | -1.98 | 0.0006 |
| 37 | Leukocyte elastase inhibitor | SERPINB1 | -1.80 | 0.0015 |
| 38 | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | ACADM | 1.73 | 0.0049 |
| 39 | Elongation factor Tu, mitochondrial | TUFM | 1.56 | 0.0008 |
| Isocitrate dehydrogenase [NADP] cytoplasmic | IDH1 | |||
| 40 | Succinate–CoA ligase [ADP-forming] subunit beta, mitochondrial | SUCLA2 | 1.50 | 0.0117 |
| 41 | Ornithine aminotransferase, mitochondrial | OAT | 1.63 | 0.0068 |
| 42 | Fascin | FSCN1 | -1.90 | 0.0422 |
| 43 | S-adenosylmethionine synthase isoform type-2 | MAT2A | -2.00 | 0.0145 |
| 44 | Aldehyde dehydrogenase X, mitochondrial | ALDH1B1 | 1.51 | 0.0007 |
| 45 | Ras GTPase-activating protein-binding protein 1 | G3BP1 | 1.51 | 0.0009 |
| 46 | Prelamin-A/C | LMNA | -1.86 | 0.0025 |
| 47 | Sorting nexin-9 | SNX9 | 1.70 | 0.0143 |
| 48 | Glycogen phosphorylase, brain form | PYGB | 1.87 | 0.0101 |
| Elongation factor 2 | EEF2 | |||
| Cytoplasmic aconitate hydratase | ACO1 | |||
| 49 | Nucleolin | NCL | -1.54 | 0.0019 |
| 50 | Heterogeneous nuclear ribonucleoprotein U-like protein 2 | HNRNPUL2 | -1.97 | 0.0092 |
| 51 | Talin-1 | TLN1 | 1.65 | 0.0253 |