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The production of OTC by S. rimosus strains with inactivated rimocidin biosynthesis loading module (RimA) compared to control strains ATCC 10970 and ATCC ∆145 kb. Four independent engineered strains with inactivated rimA were tested (∆rimA_1–∆rimA_4). Mean is plotted with error bars showing s.d. (n = 9, three independent fermentations from three biological replicates). Significance is tested by Dunnett’s T3-test, ****P < 0.0001.
