Fig 9.

Full-scan LC-MS analysis of S. rimosus ATCC 10970 (A), ATCC 10970 Δ145 kb (B) and ATCC 10970 ΔrimA production broths after acetonitrile extraction. Emergence of three new metabolites in the strain ATCC Δ145kb is visible on chromatogram B. RIM—rimocidins, OTC—oxytetracycline ([M + H]⁺_obs—see Table 2), 1—tyrobetaine-2 ([M + H]⁺_obs = 387.7), 2—longicatenamycin B ([M + H]⁺_obs = 764.9), 3—longicatenamycin C ([M + H]⁺_obs = 778.9). Compete removal of all rimocidin-related metabolites can be observed for rimA mutant, while OTC titer remain unaffected compared to ATCC 10970. Visualization with MZmine 3.0 (41).