TABLE 1.

Significantly affected^a BGCs after genome reductions in engineered S. rimosus strains^b

Significantly affected BGC	Metabolite	Fold change-log2 ratio (x vs ATCC 10970)
		ATCC Δ145 kb	ATCC Δ240 kb
BGC 22_1	Longicatenamycin B	5.36	0.88
BGC 22_2	Unknown	5.15	0.91
BGC 9—OTC	Oxytetracycline	4.7	1.7
BGC 45	Momomycin	1.73	0.79
BGC 23	Tyrobetaine-2	1.74	0.85
BGC 46	Unknown	−1.97	−1.02
BGC 5	Unknown	−2.33	/
BGC 41	Unknown	−4.09	−2.33

^a BGCs with an average gene log² fold change of >1 or <−1 in at least one mutant strain was considered significantly affected.

^b Average log² ratio—fold change of expression at 24h time point.