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Effect of spermidine on the synthesis of proteins following a shift from 37 to 7°C. Strains CAG2242/pMW119 (A), CAG2242/pMW119 in the presence of 1 mM spermidine (B), and CAG2242/pMWSAT in the presence of 1 mM spermidine (C) were labeled with [35S]methionine at 68 h postshift, and extracts were processed by two-dimensional gel electrophoresis as described in Materials and Methods. Circles indicate cold shock proteins (from left to right) CspB, CspG, and CspA.
