Fig. 2.

S. cerevisiae (A) lipid/water ²H-fractionation and (B) biomass/substrate ¹³C-fractionation for chemostat growth on fermentable sugar and nonfermentable glycerol substrates at different growth rates. Strain EXF-4126 grown with 2% glycerol (blue), 2% glucose (brown); strain FY4 grown with 0.75% glucose (orange), 0.75% galactose (yellow). Panel A bars, abundance weighted average ²ε_{fattyacid/water} of n = 3 replicate sampling timepoints; error bars are SD. Palmitic (n-C_16:0) and palmitoleic (n-C_16:1) acid ²ε_{fattyacid/water} indicated by filled and open circles, respectively (circle size, relative abundance of 16 carbon fatty acids). Panel B bars are biomass/substrate ¹³C-fractionation, mean of 3 to 6 replicate sampling timepoints; error bars are SD of 3 to 6 timepoint replicates. See details in SI Appendix, Figs. S2 and S3 and Datasets S1–S3.