Table 1.
Fatty acid/water 2H-fractionations for healthy and cancerous murine liver cells
| Sample | Metabolism | Growth (qualitative) | 2εC16:0/water (‰) | SD 2εC16:0/water (‰) | 2εC18:0/water (‰) | SD 2εC18:0/water (‰) |
|---|---|---|---|---|---|---|
| Primary hepatocyte | Respiration, healthy | Slow/no growth | −200 | 2.0 | −137 | 2.7 |
| Hepatoma (Hepa1-6) | Fermentation, cancer | Fast growth | −218 | 0.4 | −192 | 1.2 |
NADPH fluxes for de novo lipid synthesis were previously characterized by isotope tracing methods in the same or similar cell types (65). Data derive from n = 1 sample of each cell type and SD are from 3 to 4 analytical measurements.