FIG. 3.

Southern blot analysis of random insertions into the chromosome. Genomic DNA of 15 (1 to 15) representative random transposon mutants (A) and four (mal1 to mal4) mutants that present an impaired ability to utilize maltose (B) was digested with ClaI and probed with a PCR product that corresponds to the kanamycin resistance gene present in the minitransposon. Molecular sizes are indicated in kilobase pairs.