TABLE 1.

Sequence and location of random Himar1 insertion sites and potential function of the interrupted genes

Mutant	Sequence at point of insertiona	Point of insertiona	NmORFb	Potential functionb (organism)
1	ATCGACAATAATGCTACA	1489020	1582	Unknown
2	GTTGGCCATATATTGCCA	696195	708	N-acetyl-β-glucosaminidase (Vibrio furnissii)
3	GGACGATATAGAACGTGG	1202078	1274	Phosphoglyceromutase (Zymomonas mobilis)
4	AAACCGCGTATTGTACGT	973969	—c
5	ATCTTATGTATGATAATT	1743815	1799	Unknown
6	GCAGTCCGTATAAGTATT	1963690	2024	Transferrin binding protein 1 (N. meningitidis)
7	GCCGACCATACTGCCGAA	581224	608	Carbamoyl phosphate synthase small subunit (N. gonorrhoeae)
8	CGGCAAGATAGGTGATGT	1084167	1136	Homoserine O-acetyltransferase (Leptospira meyeri)
9	GAACGAAATACCCATCGA	628684	643	Lipopolysaccharide biosynthesis protein (Thermotoga maritima)
10	CGGCATTATATAGAAACC	526471	—
11	GGTTGATTGAAAAGGCGG	1972409	—
12	AAGAAGCGTAGGCTTTGC	240924	257	3-Phosphoglycerate kinase (Alcaligenes eutrophus)
13	TACCCCCATATGGTGTTG	313623	—
14	TTTTATTGTATCAATACT	933137	—
15	TAAAAGGATATAGCCTGA	1941332	2006	Unknown
16	TACCGGCGTAAGATATTG	466730	478	Serine protease homologue (Pseudomonas fluorescens)
17	CGATAATATATTATTCAT	265542	—
18	CAGATAAATACCCATACC	190812	207	Unknown
19	GGTCGATGTATTGGACTC	2103164	2155	PilG pilus assembly protein (N. meningitidis)
20	CCACAGTATAAAATTATT	33411	40	Unknown
21	GAGGGCACTATTCCAAAT	1140781	1200	Hia adhesin (Haemophilus influenzae)
22	ATCACCAATATTTCCGCC	404824	430	HrpA helicase (E. coli)
23	ACACGGTATAAGCATCCG	1174994	1248	Unknown
24	AACCGAACTATACGCCCA	200134	—
25	CAGCCTTATATTGTGCGT	1447005	1548	Unknown
26	CAAATCATTATCACTGAG	407012	432	Unknown
27	TGCACTTGTATTTGCCTT	2073103	2122	Unknown
28	ATCATAAGTAACCTTGAT	1729621	1786	Periplasmic putrescine binding protein (E. coli)
29	TTCCAATCTAAGCAGAAC	1723027	1778	Secreted protease (E. coli)
30	TTGGGGAATACTCATTCT	1458816	1557	Unknown
31	CATAGCGGTATGCATAGG	16031	20	Unknown
32	GGCGTGCATATTTATCGT	890614	915	Unknown
33	CCGAGCTGTACTTGGGCG	1104221	1153	Succinyl-CoAd synthetase β subunit (E. coli)
34	TCGTTCCGTATCCCAGTT	2095153	2147	Lactoylglutathione lyase (N. meningitidis)
35	AGACGGCATACCGCTCAG	1150268	1206	Oxidoreductase (Helicobacter pylori)
36	GTCGTCTTTATGGCGGAC	829845	849	Unknown
37	AAAGGTAGTAGTCGTAAC	538662	561	DNA adenine methylase pseudogene (N. meningitidis)
38	TTCGATAATAATTCGCAA	377284	—
39	GTGTATAATATATAACAT	182083	—
40	TAAGGATGTAGCGTCCGA	828989	848	UTP-glucose-1-phosphate uridylyltransferase (E. coli)
41	TATCATTATATTAATATG	1681423	—
42	AGGAAAACTATCCGCAGA	1577037	1660	ATP-dependent DNA helicase (E. coli)
43	CGAGGCCGTAATGGTAGA	166035	184	Unknown
44	TCTACCCATAATACGCAT	675145	688	FhaB (Bordetella pertussis)
45	CAAACAAATAGGGGACTG	33147	39	Unknown
mal1	TGGCTTCATACAAATGAC	1146010	1205	d-Lactate dehydrogenase (E. coli)
mal2	GTTCGTAGAAGTCGAAAT	2049901	2098	Maltose phosphorylase (Lactobacillus sanfranciscensis)
mal3	GAATCATGTATTTACCCC	2050315	2098	Maltose phosphorylase (L. sanfranciscensis)
mal4	TTGCGGCATACAGATAGA	2053125	2100	Maltose/H+ symporter (Daucus carota)

^aThe point of insertion of the transposon was after the dinucleotide in bold letters that was duplicated upon insertion. 

^bWhen insertions occurred into one of the 2,230 predicted ORFs from the Z2491 sequence (24), its number (NmORF) and potential function are indicated. 

^c—, insertions occurred in intergenic regions. 

^dCoA, coenzyme A.