TABLE 3.
Analysis of translation of aldB::lux fusions
Plasmid | Insert | M17
|
CDM with BCAA
|
CDM without BCAA
|
|||
---|---|---|---|---|---|---|---|
Lux activitya | Inhibition factorb | Lux activity | Inhibition factor | Lux activity | Inhibition factor | ||
pJIM1730 | RBS | 200 | 1 | 250 | 1 | 130 | 1 |
pJIM1732 | Stem II (anti-RBS/RBS) | 9.3 | 22 | 2.0 | 125 | 1.9 | 68 |
pJIM1735 | Stems I and II (tRNA-like/anti-RBS/RBS) | 1.6 | 125 | 0.49 | 510 | 0.53 | 245 |
pJIM1739 | Stems I and II, ′ORF(+1) translated | 1.2 | 167 | 0.60 | 417 | 0.31 | 419 |
pJIM1740 | Stems I and II, ′ilvA translated | 0.45 | 444 | 0.10c | >2,500 | 0.11c | >1,200 |
In kilolux per OD unit.
Ratio of luciferase activity to that obtained with pJIM1730.
Value at the threshold level.