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. 2000 Oct;182(19):5399–5408. doi: 10.1128/jb.182.19.5399-5408.2000

TABLE 3.

Analysis of translation of aldB::lux fusions

Plasmid Insert M17
CDM with BCAA
CDM without BCAA
Lux activitya Inhibition factorb Lux activity Inhibition factor Lux activity Inhibition factor
pJIM1730 RBS 200 1 250 1 130 1
pJIM1732 Stem II (anti-RBS/RBS) 9.3 22 2.0 125 1.9 68
pJIM1735 Stems I and II (tRNA-like/anti-RBS/RBS) 1.6 125 0.49 510 0.53 245
pJIM1739 Stems I and II, ′ORF(+1) translated 1.2 167 0.60 417 0.31 419
pJIM1740 Stems I and II, ′ilvA translated 0.45 444 0.10c >2,500 0.11c >1,200
a

In kilolux per OD unit. 

b

Ratio of luciferase activity to that obtained with pJIM1730. 

c

Value at the threshold level.