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A temperature gradient from 58 to 64°C was performed. (A) The Multiplex LAMP assay for gram-positive bacteria was designed to detect three bacterial species, namely, S. epidermidis (FAM), S. agalactiae (HEX), and S. aureus (CY5). In parallel, (B) the Multiplex LAMP assay for gram-negative bacteria was designed to target two bacterial species, P. aeruginosa (FAM) and E. coli (CY5).
