Table 2. Gram-positive/negative bacteria quantitative real-time PCR primer sets.

Target (gene)	Name	Sequence (5’-3’)	μM	Reference
S. epidermidis (atlE gene)	SEP_atlE_F	GGAGGAACTAATAATAAGTTAACTG	10	[28]
	SEP_atlE_R	GTCATAAACAGTTGTATATAAGCC	10
	SEP_atlE_P	FAM-CTGCTAATCGTGGTGTTGCTCAAATTAAA-BHQ1	10
S. agalactiae (sodA gene)	SAG_sip_F	GTTCCAGCAGCTAAAGAGGAAG	10	[29]
	SAG_sip_R	CCGGTGCTACTTTAGCTACTGG	10
	SAG_sip_P	HEX-CACCAGCTTCTGTTGCCGCTGAAACACCAGC-BHQ1	10
S. aureus (nuc gene)	SAU_nuc_F	CACCTGAAACAAAGCATCCTAAA	10	[30]
	SAU_nuc_R	CGCTAAGCCACGTCCATATT	10
	SAU_nuc_P	CY5-TGGTCCTGAAGCAAGTGCATTTACGA-BHQ2	10
P. aeruginosa (gyrB gene)	PAE_gyrB_F	GGCGTGGGTGTGGAAGTC	10	[31]
	PAE_gyrB_R	TGGTGGCGATCTTGAACTTCTT	10
	PAE_gyrB_P	FAM-TGCAGTGGAACGACA-BHQ1	10
E. coli (16s rRNA gene)	ECO_16s_F	CATGCCGCGTGTATGAAGAA	10	[32]
	ECO_16s_R	CGGGTAACGTCAATGAGCAAA	10
	ECO_16s_P	CY5-TATTAACTTTACTCCCTTCCTCCCCGCTGAA-BHQ2	10