Table 5. Performance comparison between the gram-positive/negative bacteria multiplex LAMP assay and qPCR for clinical samples.
| Clinical Sample | Gram-positive bacteria multiplex LAMP assay | Gram-negative bacteria multiplex LAMP assay | qPCR | ||||
|---|---|---|---|---|---|---|---|
| S. agalactiae | S. epidermidis | S. aureus | P. aeruginosa | E. coli | |||
| S. agalactiae (n = 1) | P/N | 1/0 | 0/1 | 0/1 | 0/1 | 0/1 | 1/0 |
| Sensitivity | 100 | - | - | - | - | 100 | |
| Specificity | - | 100 | 100 | 100 | 100 | - | |
| S. epidermidis (n = 1) | P/N | 0/1 | 1/0 | 0/1 | 0/1 | 0/1 | 1/0 |
| Sensitivity | - | 100 | - | - | - | 100 | |
| Specificity | 100 | - | 100 | 100 | 100 | - | |
| S. aureus (n = 4) | P/N | 0/4 | 0/4 | 4/0 | 0/4 | 0/4 | 4/0 |
| Sensitivity | - | - | 100 | - | - | 100 | |
| Specificity | 100 | 100 | - | 100 | 100 | - | |
| Negative Sample (n = 14) | P/N | 0/14 | 0/14 | 0/14 | 0/14 | 0/14 | 0/14 |
| Sensitivity | - | - | - | - | - | - | |
| Specificity | 100 | 100 | 100 | 100 | 100 | 100 | |
“P” and “N” indicate the positive and negative reactions, respectively.
LAMP: Loop-mediated isothermal amplification, qPCR: quantitative real-time PCR.